Disruption of the gene causes hypersensitivity to Ultraviolet B light and -rays in Arabidopsis; Implication of the presence of a translesion synthesis mechanism in plants

Sakamoto, Ayako; Lan, V. T. T.; Hase, Yoshihiro; Shikazono, Naoya; Matsunaga, Tsukasa*; Tanaka, Atsushi

Plant Cell, 15(9), p.2042 - 2057, 2003/09

https://doi.org/10.1105/tpc.012369

In order to investigate UV-response mechanisms in higher plants, we isolated a UV-sensitive mutant, , in . The root growth of was inhibited after UV-B irradiation under both photoreactivating and non-photoreactivating conditions. We found that chromosome 1 of was broken at least at three points, causing chromosome inversion and translocation. A gene disrupted by this rearrangement encoded the catalytic subunit of DNA polymerase (), which is thought to be involved in translesion synthesis. The seedlings were also sensitive to -rays and MMC, which are known to inhibit DNA replication. The incorporation of BrdU after UV-B irradiation was less in than in the wild type plant. These results suggest that UV-damaged DNA interrupted DNA replication in the mutant, leading to the inhibition of cell division and root elongation.

X-Ray Photoelectron and X-ray-Induced Auger Electron Spectroscopic Data, II; 4d Transition-Metals(Y,Zr,Nb,Mo,Ru)and Related Oxides

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JAERI-M 84-071, 41 Pages, 1984/04

JAERI-M-84-071.pdf:0.67MB

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